primary human ecs Search Results


5×10 5 primary human dermal microvascular ecs  (Lonza)
90
Lonza 5×10 5 primary human dermal microvascular ecs
5×10 5 Primary Human Dermal Microvascular Ecs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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5×10 5 primary human dermal microvascular ecs - by Bioz Stars, 2026-04
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human dermal microvascular endothelial cells  (BioWhittaker Molecular Applications)
90
BioWhittaker Molecular Applications human dermal microvascular endothelial cells
Human Dermal Microvascular Endothelial Cells, supplied by BioWhittaker Molecular Applications, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
human dermal microvascular endothelial cells - by Bioz Stars, 2026-04
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primary human artery ecs  (European Collection of Authenticated Cell Cultures)
90
European Collection of Authenticated Cell Cultures primary human artery ecs
Primary Human Artery Ecs, supplied by European Collection of Authenticated Cell Cultures, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
primary human artery ecs - by Bioz Stars, 2026-04
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primary human ecs  (Angiocrine)
90
Angiocrine primary human ecs
Primary Human Ecs, supplied by Angiocrine, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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primary human ecs - by Bioz Stars, 2026-04
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primary human umbilical arterial ecs (huaec  (Vec Technologies)
90
Vec Technologies primary human umbilical arterial ecs (huaec
Primary Human Umbilical Arterial Ecs (Huaec, supplied by Vec Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary human umbilical arterial ecs (huaec/product/Vec Technologies
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primary human umbilical arterial ecs (huaec - by Bioz Stars, 2026-04
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human pulmonary endothelial cells  (Lonza)
90
Lonza human pulmonary endothelial cells
Human Pulmonary Endothelial Cells, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human pulmonary endothelial cells - by Bioz Stars, 2026-04
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primary human umbilical ecs  (Lonza)
90
Lonza primary human umbilical ecs
FABP4 knockdown reduces vascular sprout elongation and endothelial cell viability. <t>Human</t> <t>umbilical</t> vein endothelial cells (EC) were transfected with non-targeting control siRNA or two different sequences of siRNA targeting FABP4 (20 n m , pooled or single as indicated). To determine vascular sprouting, transfected or <t>ECs</t> were grown as spheroids in hanging drops and embedded in matrigel, and sprout number and length were quantified after 48 h. n =4 ( a ). To determine migration, scratched confluent monolayers were monitored for % wound closure over time ( b ). To measure cell number as an indicator for proliferation, ECs transfected with pooled siRNA sequences #1 and #2 targeting FABP4 were grown for 72 h, trypsinized and counted. n =3 ( c ). Cell viability was determined in the same samples based on cell membrane permeability for trypan blue. n =4 ( d ). * P <0.05; ** P <0.01; *** P <0.001. Error bars, s.d.
Primary Human Umbilical Ecs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary human umbilical ecs/product/Lonza
Average 90 stars, based on 1 article reviews
primary human umbilical ecs - by Bioz Stars, 2026-04
90/100 stars
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all human primary vascular ecs  (Lonza)
90
Lonza all human primary vascular ecs
FABP4 knockdown reduces vascular sprout elongation and endothelial cell viability. <t>Human</t> <t>umbilical</t> vein endothelial cells (EC) were transfected with non-targeting control siRNA or two different sequences of siRNA targeting FABP4 (20 n m , pooled or single as indicated). To determine vascular sprouting, transfected or <t>ECs</t> were grown as spheroids in hanging drops and embedded in matrigel, and sprout number and length were quantified after 48 h. n =4 ( a ). To determine migration, scratched confluent monolayers were monitored for % wound closure over time ( b ). To measure cell number as an indicator for proliferation, ECs transfected with pooled siRNA sequences #1 and #2 targeting FABP4 were grown for 72 h, trypsinized and counted. n =3 ( c ). Cell viability was determined in the same samples based on cell membrane permeability for trypan blue. n =4 ( d ). * P <0.05; ** P <0.01; *** P <0.001. Error bars, s.d.
All Human Primary Vascular Ecs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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all human primary vascular ecs - by Bioz Stars, 2026-04
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primary human umbilical vein ecs  (Shanghai Biotechnology Co Ltd)
90
Shanghai Biotechnology Co Ltd primary human umbilical vein ecs
FABP4 knockdown reduces vascular sprout elongation and endothelial cell viability. <t>Human</t> <t>umbilical</t> vein endothelial cells (EC) were transfected with non-targeting control siRNA or two different sequences of siRNA targeting FABP4 (20 n m , pooled or single as indicated). To determine vascular sprouting, transfected or <t>ECs</t> were grown as spheroids in hanging drops and embedded in matrigel, and sprout number and length were quantified after 48 h. n =4 ( a ). To determine migration, scratched confluent monolayers were monitored for % wound closure over time ( b ). To measure cell number as an indicator for proliferation, ECs transfected with pooled siRNA sequences #1 and #2 targeting FABP4 were grown for 72 h, trypsinized and counted. n =3 ( c ). Cell viability was determined in the same samples based on cell membrane permeability for trypan blue. n =4 ( d ). * P <0.05; ** P <0.01; *** P <0.001. Error bars, s.d.
Primary Human Umbilical Vein Ecs, supplied by Shanghai Biotechnology Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/primary human umbilical vein ecs/product/Shanghai Biotechnology Co Ltd
Average 90 stars, based on 1 article reviews
primary human umbilical vein ecs - by Bioz Stars, 2026-04
90/100 stars
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human primary vascular ecs haec  (Lonza)
90
Lonza human primary vascular ecs haec
FABP4 knockdown reduces vascular sprout elongation and endothelial cell viability. <t>Human</t> <t>umbilical</t> vein endothelial cells (EC) were transfected with non-targeting control siRNA or two different sequences of siRNA targeting FABP4 (20 n m , pooled or single as indicated). To determine vascular sprouting, transfected or <t>ECs</t> were grown as spheroids in hanging drops and embedded in matrigel, and sprout number and length were quantified after 48 h. n =4 ( a ). To determine migration, scratched confluent monolayers were monitored for % wound closure over time ( b ). To measure cell number as an indicator for proliferation, ECs transfected with pooled siRNA sequences #1 and #2 targeting FABP4 were grown for 72 h, trypsinized and counted. n =3 ( c ). Cell viability was determined in the same samples based on cell membrane permeability for trypan blue. n =4 ( d ). * P <0.05; ** P <0.01; *** P <0.001. Error bars, s.d.
Human Primary Vascular Ecs Haec, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human primary vascular ecs haec/product/Lonza
Average 90 stars, based on 1 article reviews
human primary vascular ecs haec - by Bioz Stars, 2026-04
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human primary microvascular ecs hmdecs  (Lonza)
90
Lonza human primary microvascular ecs hmdecs
FABP4 knockdown reduces vascular sprout elongation and endothelial cell viability. <t>Human</t> <t>umbilical</t> vein endothelial cells (EC) were transfected with non-targeting control siRNA or two different sequences of siRNA targeting FABP4 (20 n m , pooled or single as indicated). To determine vascular sprouting, transfected or <t>ECs</t> were grown as spheroids in hanging drops and embedded in matrigel, and sprout number and length were quantified after 48 h. n =4 ( a ). To determine migration, scratched confluent monolayers were monitored for % wound closure over time ( b ). To measure cell number as an indicator for proliferation, ECs transfected with pooled siRNA sequences #1 and #2 targeting FABP4 were grown for 72 h, trypsinized and counted. n =3 ( c ). Cell viability was determined in the same samples based on cell membrane permeability for trypan blue. n =4 ( d ). * P <0.05; ** P <0.01; *** P <0.001. Error bars, s.d.
Human Primary Microvascular Ecs Hmdecs, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/human primary microvascular ecs hmdecs/product/Lonza
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human primary microvascular ecs hmdecs - by Bioz Stars, 2026-04
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FABP4 knockdown reduces vascular sprout elongation and endothelial cell viability. Human umbilical vein endothelial cells (EC) were transfected with non-targeting control siRNA or two different sequences of siRNA targeting FABP4 (20 n m , pooled or single as indicated). To determine vascular sprouting, transfected or ECs were grown as spheroids in hanging drops and embedded in matrigel, and sprout number and length were quantified after 48 h. n =4 ( a ). To determine migration, scratched confluent monolayers were monitored for % wound closure over time ( b ). To measure cell number as an indicator for proliferation, ECs transfected with pooled siRNA sequences #1 and #2 targeting FABP4 were grown for 72 h, trypsinized and counted. n =3 ( c ). Cell viability was determined in the same samples based on cell membrane permeability for trypan blue. n =4 ( d ). * P <0.05; ** P <0.01; *** P <0.001. Error bars, s.d.

Journal: Oncogene

Article Title: Antiangiogenic and tumour inhibitory effects of downregulating tumour endothelial FABP4

doi: 10.1038/onc.2016.256

Figure Lengend Snippet: FABP4 knockdown reduces vascular sprout elongation and endothelial cell viability. Human umbilical vein endothelial cells (EC) were transfected with non-targeting control siRNA or two different sequences of siRNA targeting FABP4 (20 n m , pooled or single as indicated). To determine vascular sprouting, transfected or ECs were grown as spheroids in hanging drops and embedded in matrigel, and sprout number and length were quantified after 48 h. n =4 ( a ). To determine migration, scratched confluent monolayers were monitored for % wound closure over time ( b ). To measure cell number as an indicator for proliferation, ECs transfected with pooled siRNA sequences #1 and #2 targeting FABP4 were grown for 72 h, trypsinized and counted. n =3 ( c ). Cell viability was determined in the same samples based on cell membrane permeability for trypan blue. n =4 ( d ). * P <0.05; ** P <0.01; *** P <0.001. Error bars, s.d.

Article Snippet: Primary human umbilical ECs were purchased from Lonza, Visp, Switzerland and cultured in endothelial growth medium 2 (Lonza).

Techniques: Knockdown, Transfection, Control, Migration, Membrane, Permeability

FABP4 knockdown regulates fatty acid metabolism enzymes and increases fatty acid oxidation. Human umbilical vein endothelial cells (EC) were transfected with non-targeting control siRNA or two different sequences of siRNA targeting FABP4 (20 n m ) and mRNA expression of FABP4 and PPARG were determined and are expressed relative to β-actin (ACTB). n =5 ( a, b ). Protein levels of FABP4, phosphorylated HSL (Serine 565, p565, inhibitory; Serine 660, p660, activating) and total HSL were analysed in response to FABP4 knockdown by immunoblot analysis, using β-actin as a loading control. Band densitometry analysis of phosphorylated HSL was carried out relative to total HSL. n =3 ( c ). To measure lipid droplet accumulation, transfected ECs were exposed to media containing BSA or oleic acid (OA, serving as a positive control) conjugated to BSA for 16 h prior to staining lipid droplets with the fluorescent dye LD540 and measuring signal intensity in the fluorescein isothyanate (FITC) channel by FACS analysis. n =3 ( d ). Fatty acid uptake and oxidation were measured by detecting intracellular 14 C or 14 CO 2 derived from [U14C]-labelled OA in the cell culture media. n =4 ( e–g ). FAO rates were corrected for the levels of FAs taken up into the cells. Etomoxir (50 μ m , Eto) or dimethylsulphoxide (DMSO) were added 16 h after the transfection to inhibit fatty acid oxidation n =3 ( g ) * P <0.05; ** P <0.01; *** P <0.001. Error bars, s.d.

Journal: Oncogene

Article Title: Antiangiogenic and tumour inhibitory effects of downregulating tumour endothelial FABP4

doi: 10.1038/onc.2016.256

Figure Lengend Snippet: FABP4 knockdown regulates fatty acid metabolism enzymes and increases fatty acid oxidation. Human umbilical vein endothelial cells (EC) were transfected with non-targeting control siRNA or two different sequences of siRNA targeting FABP4 (20 n m ) and mRNA expression of FABP4 and PPARG were determined and are expressed relative to β-actin (ACTB). n =5 ( a, b ). Protein levels of FABP4, phosphorylated HSL (Serine 565, p565, inhibitory; Serine 660, p660, activating) and total HSL were analysed in response to FABP4 knockdown by immunoblot analysis, using β-actin as a loading control. Band densitometry analysis of phosphorylated HSL was carried out relative to total HSL. n =3 ( c ). To measure lipid droplet accumulation, transfected ECs were exposed to media containing BSA or oleic acid (OA, serving as a positive control) conjugated to BSA for 16 h prior to staining lipid droplets with the fluorescent dye LD540 and measuring signal intensity in the fluorescein isothyanate (FITC) channel by FACS analysis. n =3 ( d ). Fatty acid uptake and oxidation were measured by detecting intracellular 14 C or 14 CO 2 derived from [U14C]-labelled OA in the cell culture media. n =4 ( e–g ). FAO rates were corrected for the levels of FAs taken up into the cells. Etomoxir (50 μ m , Eto) or dimethylsulphoxide (DMSO) were added 16 h after the transfection to inhibit fatty acid oxidation n =3 ( g ) * P <0.05; ** P <0.01; *** P <0.001. Error bars, s.d.

Article Snippet: Primary human umbilical ECs were purchased from Lonza, Visp, Switzerland and cultured in endothelial growth medium 2 (Lonza).

Techniques: Knockdown, Transfection, Control, Expressing, Western Blot, Positive Control, Staining, Derivative Assay, Cell Culture